en عمومى Human Genetics پژوهشي Research <span style="font-size:11pt"><span style="line-height:150%"><span calibri="" style="font-family:"><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Background: As the most common inflammatory arthritis worldwide, rheumatoid arthritis (RA) has been shown in numerous studies to be significantly influenced by long non-coding RNAs (lncRNAs), particularly in terms of disease onset and progression. This research explores the possible association between <i>ANRIL</i> (rs4977574) polymorphisms and susceptibility to RA. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:150%"><span calibri="" style="font-family:"><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Methods: This study comprised 150 confirmed cases of RA alongside 150 matched healthy controls. Genotyping of DNA was carried out using the tetra-primer amplification refractory mutation system polymerase chain reaction (ARMS-PCR).</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:150%"><span calibri="" style="font-family:"><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Results: Based on multivariate logistic regression, carriers of the AG genotype had a 7.13‑fold higher risk of developing RA compared to those with the AA genotype.</span></span></span> <span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Conversely, the <a name="_Hlk200048263">GG genotype </a>was associated with a substantial 0.18-fold reduction in RA risk compared to AA. Additionally, the <i>ANRIL</i> rs4977574 variant was shown to increase RA risk across recessive </span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">(OR = 1.52, 95% CI = 1.06-2.19, P&nbsp;= 0.022)</span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">, dominant </span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">(OR = 2.49, 95% CI = 1.50-4.13, P&nbsp;= 0.0003)</span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">, and over-dominant </span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">(OR = 8.19, 95% CI = 4.086-16.4, P&nbsp;< 0.0000001) </span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">genetic models. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:150%"><span calibri="" style="font-family:"><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Conclusion: This study established a connection between <i>ANRIL</i> genetic variation and an elevated risk of RA within the Iranian population.</span></span></span></span></span></span> Rheumatoid arthritis, ANRIL Long non-coding RNA, Tetra-primer ARMS-PCR, Single Nucleotide Polymorphism 0 0 http://humangeneticsgenomics.ir/browse.php?a_code=A-10-403-1&slc_lang=en&sid=1 Ali Karimizadeh genetics.ali@gmail.com 10031947532846001194 10031947532846001194 No Department of Biology, Zarg.C., Islamic Azad University, Zarghan, Iran Mohammad Javad Mokhtari mj.mokhtari@gmail.com 10031947532846001195 10031947532846001195 Yes Department of Biology, Zarg.C., Islamic Azad University, Zarghan, Iran