en عمومى Human Genetics پژوهشي Research <span style="font-size:11pt"><span style="line-height:200%"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Abstract</span></span></span></b><br> Background: Extremely low-frequency magnetic field &nbsp;(ELF-MF) is &nbsp;widespread environmental factors that &nbsp;may influence cellular behavior through non-thermal mechanisms. Recent evidence suggests that electromagnetic exposure can modulate gene expression and microRNA (miRNA) profiles in cancer cells. The aim of this research is &nbsp;the 50 Hz ELF-MF effect on the expression of miR-124, miR-195, and their target gene CDK6 in the 5637 bladder cancer cell line.<br> Methods: 5637 bladder cancer cells were cultured under standard conditions and exposed to ELF-MF for 6 hours per day over three consecutive days. The viability &nbsp;of cell was evaluated by MTT method. Total RNA extracted, and the change of expression of miR-124, miR-195, and CDK6 were quantified by quantitative real-time PCR.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Results: ELF-MF exposure could &nbsp;significant reduced &nbsp;of the viability &nbsp;of cell . The greatest reduction observed at 4 mT. Quantitative analysis revealed significant upregulation of miR-124 and miR-195 following magnetic field exposure, while CDK6 expression was significantly downregulated. Spearman correlation results demonstrated a negative correlation between miR-124/miR-195 and CDK6 expression and a positive correlation between miR-124 and miR-195.</span></span></span><br> Conclusion: Exposure to ELF-MF modulates miRNA expression and suppresses CDK6 , suggesting that magnetic fields can &nbsp;inhibit cell proliferation through miRNA-mediated regulation of the cell cycle. These findings provide insight into the interaction between physical environmental factors and gene regulatory networks in cancer biology.</span></span></span></span><br> &nbsp; Bladder cancer, Extremely low-frequency magnetic field (ELF-MF), miR-124, miR-195, CDK6 0 0 http://humangeneticsgenomics.ir/browse.php?a_code=A-10-323-2&slc_lang=en&sid=1 Soheila Abdi abdi.soheila@gmail.com 10031947532846001196 10031947532846001196 No Department of Physics, ShQ. C., Islamic Azad University, Shahr-e Qods, Iran Behnaz Gharekhanlou behnaz.gharekhanlou@iau.ac.ir 10031947532846001197 10031947532846001197 Yes Department of Electrical Engineering, CT.C., Islamic Azad University, Tehran, Iran Sepideh Gholamrezaie Sepidehgholamrezaie@gmail.com 10031947532846001198 10031947532846001198 No Farhikhtegan Medical Convergence sciences Research Center, Farhikhtegan Hospital. TeMS.C., Islamic Azad University, Tehran, Iran