The capability of cancer cells including tumor initiation, maintenance, and extension is associated with cancer stem cells. The substantial features of CSCs are self - renewal and pluripotency. Therapeutic resistance of CSCs can result in cancer recurrence and failure of cancer treatment. Isolation and characterization of CSCs are required for targeted cancer therapies. CSCs can be distinguished and separated by surface markers. In this review, after definition of CSC hypothesis, different markers of CSC are introduced. The information about CSC markers provides promise for better isolation and more effective CSC-targeting therapeutic in future.