Human Genetics and Genomics
Journal of Human Genetics and Genomics
jhgg
Medical Sciences
http://humangeneticsgenomics.ir
1
admin
2588-6479
2588-6479
8
10.61186/jhgg
14
8888
13
en
jalali
1400
9
1
gregorian
2021
12
1
5
2
online
1
fulltext
en
Evaluation of changes in expression of muc19 and muc21 genes in colorectal cancer tissues
تخصصي
Gene expression
پژوهشي
Research
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<td style="padding: 0in 12px; text-align: justify;" valign="top"><span style="font-family:Tahoma;"><span style="font-size:14px;"><span style="line-height:115%"><span style="line-height:115%">Colorectal cancer is the third most common cause of cancer deaths in the world. Mucins are large glycoproteins that are expresses by epithelial cells in tubular organs. They recreate important functions in the regeneration and differentiation of epithelium, cell adhesion, immune response, and cellular signaling. In this study, mucin-19 and 21 gene expression were investigated in cancerous tumor samples, and tumor margins in patients with colorectal cancer. The purpose of working on these genes is their potential biomarker for the diagnosis of colorectal cancer. After extraction of RNA from 30 pairs of tumor and peripheral tumors of the colorectal tumor, cDNA was synthesized using a thermosetting kit, and RT-PCR was used to find the optimum temperature and specificity of primers. Then, real-time qPCR was performed to examine the difference between the gene expression of mucin on the tumor and tumor margins. This study showed an increase in the expression of mucin 19 in colorectal cancer tissue samples with tumor margins. </span></span><br>
<span style="line-height:115%"><b><span style="line-height:115%">Conclusion</span></b><span style="line-height:115%">: The upregulation levels of mucin 19 gene can correlate with colorectal cancer progression. Therefore, Mucin 19 may be used as a biomarker for detecting colorectal cancer development. </span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><span dir="RTL" lang="AR-SA" style="font-size:9.0pt"><span style="line-height:107%"><span style="font-family:CapitoliumNews-Regular"></span></span></span></span></span></span></td>
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Colorectal cancer, Biomarker, Mucins, Muc19 gene
0
0
http://humangeneticsgenomics.ir/browse.php?a_code=A-10-354-1&slc_lang=en&sid=1
Yasamin
Takhsha
bmshyn@yahoo.com
1003194753284600364
1003194753284600364
No
Tehran Medical Sciences, Islamic Azad University
Maliheh
Entezari
mentezari@iautmu.ac.ir
1003194753284600365
1003194753284600365
Yes
Tehran Medical Sciences, Islamic Azad University
Hossein
Fahimi
fahimihossein@yahoo.com
1003194753284600366
1003194753284600366
No
Tehran Medical Sciences, Islamic Azad University
Hossein
Dehghani
dehghanih@yahoo.com
1003194753284600367
1003194753284600367
No
Tehran Medical Sciences, Islamic Azad University