Human Genetics and Genomics
Journal of Human Genetics and Genomics
jhgg
Medical Sciences
http://humangeneticsgenomics.ir
1
admin
2588-6479
2588-6479
8
10.61186/jhgg
14
8888
13
en
jalali
1401
2
1
gregorian
2022
5
1
6
1
online
1
fulltext
en
Investigating virulence gene and sulfonamide antibiotic resistance in Proteus mirabilis isolated from urinary tract infections in Iran
تخصصي
Association study
پژوهشي
Research
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:115%"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Background and purpose: </span></span></span></b><i><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Proteus mirabilis</span></span></span></i><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> is one of the most important causes of urinary tract infection (UTI) and an opportunistic pathogen that can cause infection, especially in the urinary tract and bladder, pyelonephritis or kidney stones, especially in people with catheters or people with abnormalities of urinary system. The pathogenicity of this bacterium is caused by several genes, and sulfonamides prevent the growth of these bacteria by inhibiting DNA synthesis. The purpose of this study is to investigate the pathogenic genes that cause sulfonamide antibiotic resistance in <i>Proteus mirabilis</i> isolated from urinary infections in Iran.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:115%"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Methods: </span></span></span></b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">In this study, biochemical tests were performed to confirm the identity of <i>Proteus mirabilis</i> bacteria and DNA extraction, and then multiplex PCR tests were performed to identify pathogenicity genes Fal and Urea and antibiotic resistance genes Sul1 and Sul2 using specific primers. </span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:115%"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Results: </span></span></span></b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">In this research, biochemical tests confirmed the identity of the bacteria and the target strain and multiple resistance of the <i>Proteus mirabilis</i> strain was observed to several different types of antibiotics. In addition, Multiplex PCR verified that more than 90% of the isolated strains had pathogenic genes (91.7% Fal and 95% Urea gene) and more than 50% of the strains had antibiotic resistance genes (51.7% Sul1 and 56.7% Sul2).</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:115%"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Conclusion: </span></span></span></b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Considering the excessive use of antibiotics worldwide and the increasing spread of antibiotic resistance, it is better to check the pathogenic genes and their resistance to the antibiotics during the outbreak of the disease, consequently, the use of the antibiotics and their side effects must be reduced and to prevent the spread of antibiotic resistance.</span></span></span></span></span></span></span></span></span>
Proteus mirabilis, Multiplex PCR, Sul1, Fal, Urea, Sul2
0
0
http://humangeneticsgenomics.ir/browse.php?a_code=A-10-356-1&slc_lang=en&sid=1
Hussain
Mohammad Kazem
hussain58mohammed@gmail.com
1003194753284600427
1003194753284600427
No
Department of Microbiology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Zahra
Shafiei
shafieezahra641@gmail.com
1003194753284600428
1003194753284600428
Yes
Assistant Professor of Microbiology, Department of Biology, School of Science and Agriculture, Islamic Azad University, Roudehen Branch, Tehran, Iran
Farzaneh
Hosseini
farzaneh953@yahoo.com
1003194753284600429
1003194753284600429
No
Assistant Professor of Microbiology, Department of Microbiology, Faculty of Biological Sciences, Islamic Azad University, Tehran North Branch, Tehran, Iran