Human Genetics and Genomics
Journal of Human Genetics and Genomics
jhgg
Medical Sciences
http://humangeneticsgenomics.ir
1
admin
2588-6479
2588-6479
8
10.61186/jhgg
14
8888
13
en
jalali
1401
2
1
gregorian
2022
5
1
6
1
online
1
fulltext
en
Hsa-mir-556-5p: a prognostic factor in oral squamous cell carcinoma
عمومى
Human Genetics
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Background</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">: Around 5% of tumors develop in the head and neck area, with nearly 50% of those appearing in the oral cavity. Oral cancer ranks as the sixth leading cause of cancer-related deaths. These figures illustrate the severity of the disease and emphasize the importance of raising awareness and providing early screening to detect and manage it. The treatment and prognosis of oral squamous cell carcinoma (OSCC) remain a significant challenge. This highlights the need for more effective treatments and strategies to improve the prognosis for individuals diagnosed with OSCC. In recent years, there has been significant progress in using microRNAs (miRNAs) as diagnostic and prognostic markers for cancer, making them a highly useful and valuable tool. </span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Methods</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">: For this research, we cultured and maintained cell lines for hypopharyngeal cancer (FaDu), oral cancer (Cal 27), and PDL cells. We then used quantitative real-time polymerase chain reaction (qRT-PCR) to confirm the expression levels of the potential biomarker, has-miR-556-5p </span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Results</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">: During the discovery phase, we identified hsa-miR-556-5p as being differentially expressed, with a statistically significant increase in its expression level. More recently, we used real-time PCR to confirm that hsa-miR-556-5p is markedly up-regulated in oral squamous cell carcinoma (OSCC). Our study suggests that hsa-miR-556-5p has the potential to be a new and innovative biomarker for OSCC.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Conclusions</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">: The candidate miRNA can be chosen as a promising biomarker for predicting patient outcomes and even for early detection of the disease in clinical settings.</span></span></span></span></span></span><br>
Oral squamous cell carcinoma, Hsa-miR-556-5p, real-time PCR
0
0
http://humangeneticsgenomics.ir/browse.php?a_code=A-10-362-1&slc_lang=en&sid=1
Behnaz
Raei
1003194753284600450
1003194753284600450
No
Department of Genetic, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
Yousef
Seyedena
1003194753284600451
1003194753284600451
No
Department of Genetic, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
Mojgan
Alaeddini
1003194753284600452
1003194753284600452
No
Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
Nazanin
Hosseinkhan
1003194753284600453
1003194753284600453
No
Endocrine Research Center, The Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran
Mehrdad
Hashemi
1003194753284600454
1003194753284600454
Yes
Farhikhtegan Medical Convergence Sciences Research Center, Farhikhtegan Hospital, Tehran, Iran