en عمومى Human Genetics پژوهشي Research <span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Background:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""> The uncontrolled growth of the cells in the breast tissue causes breast cancer. About 1.38 new cases of breast cancer are diagnosed worldwide every year. Breast cancer is the most common cancer among women in the United States, with over 266,000 new cases expected for the year 2018. The evolution of breast cancer is a multi-stage process that involves genetic, epigenetic, and environmental factors that may interfere with the main settings of oncogenes and tumor suppressor genes, leading to the activation of signaling pathways associated with cancer that one of the most important of these pathways is the apoptosis process.</span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""> The Bax gene also acts as an apoptotic stimulant. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Method:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""> Due to the importance of this gene in the apoptotic process, in this research, the expression level of the Bax gene under treatment of cisplatin and Centaurea behen agents for 24 hours and 48 hours was investigated using the Real-time-PCR method. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Results:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""> The results obtained illustrate that the expression level of this gene under treatment with the cisplatin and Centaurea behen has increased compared to the non-treatment state, so this expression increase showed a significant difference between the samples group and control group (P<0.05).</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></span></b> <span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">We have shown that the Centaurea behen extract and cisplatin could induce apoptosis in the MCF-7 cell line, over-expressing of the Bax gene.</span></span></span></span></span></span><br> &nbsp; apoptosis, breast cancer, Bax, cisplatin, Centaurea behen, quantitative-PCR method 0 0 http://humangeneticsgenomics.ir/browse.php?a_code=A-10-377-1&slc_lang=en&sid=1 Maliheh Entezari mentezari@iautmu.ac.ir 1003194753284600928 1003194753284600928 Yes Department of Genetics, Faculty of Advanced Science and Technology ,Tehran medical sciences, Islamic Azad University, ,Tehran, Iran Somaieh Sheikhan ss.sheikhan@gmail.com 1003194753284600929 1003194753284600929 No Department of Genetics, Faculty of Biological Sciences,Tonekabon Branch, Islamic Azad University, Tonekabon, Iran Zeinab Khazaei Koohpar dz.khazaei@gmail.com 1003194753284600930 1003194753284600930 No Department of Cellular and Molecular Biology, Faculty of Biological Sciences,Tonekabon Branch, Islamic Azad University, Tonekabon, Iran