en تخصصي Gene expression پژوهشي Research <span style="font-size:12pt"><span style="line-height:200%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Background: </b>Gastric cancer is one of the most common types of malignancies with a high mortality rate. Treatment approaches for this disease include surgery and chemotherapy, which are associated with high complications and low efficacy. Therefore, finding new therapeutic or adjuvant approaches is of great importance. This study aimed to investigate the effect of resveratrol (RSV) on the AGS gastric cancer cell line.</span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:200%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Methods</b>: After preparing and culturing AGS cells, they were treated with different concentrations of RSV for 48h. Then, cell viability and apoptosis rate were studied using MTT assay and flow cytometry technique, respectively. Moreover, after RNA extraction, cDNA synthesis and primer design, the expressions of <i>bax</i>, <i>bcl-2</i>, <i>casp3</i> and <i>p53</i> genes were studied using RT-PCR technique.</span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:200%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Results</b>: RSV showed cytotoxic effects on AGS cells in the MTT assay and decreased cell viability. In addition, cells exposed to 50 and 100 &mu;g/mL of RSV showed a significantly higher apoptosis rate compared to control cells. Finally, RSV overexpressed the <i>bax</i>, <i>p53</i>, and <i>casp3</i> genes and downregulated the <i>bcl-2</i> in AGS cells.</span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:200%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Conclusion</b>: Resveratrol is able to induce anticancer effects in gastric cancer by inducing apoptosis through the activation of mitochondrial apoptotic pathway and has the potential to be used as a therapeutic or adjuvant treatment option in gastric cancer patients.</span></span></span></span><br> &nbsp; Resveratrol, Gastric cancer, Apoptosis, Gene, Expression 0 0 http://humangeneticsgenomics.ir/browse.php?a_code=A-10-369-3&slc_lang=en&sid=1 Hakimeah Ghorbani ghorbani96@yahoo.com 1003194753284600931 1003194753284600931 No Plant Science Department, University of Tabriz, 51666 Tabriz, Iran. Kiumarth Amini Drkiumarthamini1364@gmail.com 1003194753284600932 1003194753284600932 No Department of Clinical Pharmacy, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran. Behdokht Jamali Behdokhtjamali@gmail.com 1003194753284600933 1003194753284600933 Yes Department of microbiology, Kherad Institute of Higher Education, Bushehr, Iran. Siroos Tarighi siroosta2@gmail.com 1003194753284600934 1003194753284600934 No Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.