en عمومى Human Genetics پژوهشي Research <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Nanoparticle (NP) based systems are developed to enhance drug delivery, reducing the toxic effect of medicine molecules. iron oxide nanoparticles are used for cancer treatment and have several advantages. For example, the ability to target specific locations and reduced side effects. This study aimed to investigate the therapeutic effect of colorectal cancer cells treated with biological synthesized Fe2O3 NPs with <i>Prosopis farcta</i> on polylactic acid scaffolds. </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span style="font-size:12.0pt"></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Materials and Methods</span></span></b></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Colorectal cancer cell line HT-29 and normal cells HEK-293 were cultured on PLA scaffold and treated with biological synthesized Fe2O3 NPs for 48h. The influence of the treatment on the cells was examined by MTT and flow cytometry. To study the therapeutic mechanism of the Fe2O3 <i>Bax</i>, <i>Bcl-2</i> and were detected by real-time polymerase chain reaction (RT-PCR). </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span style="font-size:12.0pt"></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results</span></span></b></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Fe2O3 NPs treatment (112 &micro;g/ml) proved to have a good anticancer effect through the inhibition of tumoral cell proliferation and cellular growth significantly. It was shown that Fe2O3 could cause cellular necrosis, induce cellular apoptosis with reduced effects on normal colorectal cells.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span style="font-size:12.0pt"></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusion</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Our data showed that biological synthesized Fe2O3 increases significantly reduction of cancer cell viability with reduced side effect on normal cells. </span></span></span></span></span></span><br> &nbsp; Fe2O3 NPs, Colorectal Cancer, Apoptosis, Bax, Bcl2. 0 0 http://humangeneticsgenomics.ir/browse.php?a_code=A-10-394-1&slc_lang=en&sid=1 Saina Ghasemi sainaghasemi76@gmail.com 10031947532846001080 10031947532846001080 No Department of Biology, CTC, Islamic Azad University, Tehran, Iran Zeinab Piravar saba.piravar@gmail.com 10031947532846001081 10031947532846001081 Yes Department of Biology, CTC, Islamic Azad University, Tehran, Iran Mina Ramezani mina.ramezani@gmail.com 10031947532846001082 10031947532846001082 No Department