en تخصصي Gene expression پژوهشي Research <div style="text-align: justify;"> <div> <table align="left" hspace="0" vspace="0"> <tbody> <tr> <td style="padding-top:0in; padding-right:12px; padding-bottom:0in; padding-left:12px" valign="top"><span style="font-size:14px;"><span style="font-family:Tahoma;"><span style="line-height:115%"><b><span style="line-height:115%">Background: &nbsp;</span></b><span style="line-height:115%">The Islets of Langerhans include Alpha, Beta, Delta, and Epsilon cells whose secret hormones play important roles in glucose metabolism as well as some physiological processes in our own body. </span></span><br> <span style="line-height:115%"><b><span style="line-height:115%">Method:</span></b><span style="line-height:115%"> In this study, we selected a microarray row data of transplanted pancreatic Islets from Gene Expression Omnibus (GEO) database. The row data of 10 individual samples was analyzed with R programing software. Top expressed genes in human pancreatic islets were chosen and the gene stable IDs were returned to gene names and descriptions by BioMart tools. The selected genes were categorized into biological processes by Protein Analysis Through Evolutionary Relationships (PANTHER) online database. Also, sub-cellular localization of their proteins was investigated by the protein atlas database.</span></span><br> <span style="line-height:115%"><b><span style="line-height:115%">Results:</span></b><span style="line-height:115%"> The results showed that the quality controls of all 10 individual samples of microarray chips such as signal intensity and uniformity of the images were passed. From 336 genes, 284 proteins were classified by PANTHER online database. They are categorized into &ldquo;Translational proteins&rdquo;, &ldquo;Metabolism enzymes&rdquo;, and &ldquo;Protein modifying enzymes&rdquo; biological processes, respectively. </span></span><br> <span style="line-height:115%"><b><span style="line-height:115%">Conclusion:</span></b><span style="line-height:115%"> In this study, we presented 500 top-ranked expressed genes in human pancreatic islets. We also represented calcification and sub-cellular localization of these high expressed genes in separate supplementary files. Research data can be used for pancreatic research as well as potential drug design for type I diabetes or pancreatic cancers.</span></span></span></span></td> </tr> </tbody> </table> </div> </div> Microarray data analysis, Islets of Langerhans, Gene expression profile 0 0 http://humangeneticsgenomics.ir/browse.php?a_code=A-10-349-1&slc_lang=en&sid=1 Hamed Dabiri hdabiri@hotmail.com 1003194753284600360 1003194753284600360 No 1Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. Majid Sadeghizadeh sadeghma@modares.ac.ir 1003194753284600361 1003194753284600361 Yes 1Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. Vahab Ziaei ziaei.vahab@gmail.com 1003194753284600362 1003194753284600362 No National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran. Zahra Moghadasi z.moghadasi35@yahoo.com 1003194753284600363 1003194753284600363 No National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.