Human Genetics and Genomics

en Hsa-mir-556-5p: a prognostic factor in oral squamous cell carcinoma عمومى Human Genetics پژوهشي Research Background: Around 5% of tumors develop in the head and neck area, with nearly 50% of those appearing in the oral cavity. Oral cancer ranks as the sixth leading cause of cancer-related deaths. These figures illustrate the severity of the disease and emphasize the importance of raising awareness and providing early screening to detect and manage it. The treatment and prognosis of oral squamous cell carcinoma (OSCC) remain a significant challenge. This highlights the need for more effective treatments and strategies to improve the prognosis for individuals diagnosed with OSCC. In recent years, there has been significant progress in using microRNAs (miRNAs) as diagnostic and prognostic markers for cancer, making them a highly useful and valuable tool. Methods: For this research, we cultured and maintained cell lines for hypopharyngeal cancer (FaDu), oral cancer (Cal 27), and PDL cells. We then used quantitative real-time polymerase chain reaction (qRT-PCR) to confirm the expression levels of the potential biomarker, has-miR-556-5p Results: During the discovery phase, we identified hsa-miR-556-5p as being differentially expressed, with a statistically significant increase in its expression level. More recently, we used real-time PCR to confirm that hsa-miR-556-5p is markedly up-regulated in oral squamous cell carcinoma (OSCC). Our study suggests that hsa-miR-556-5p has the potential to be a new and innovative biomarker for OSCC. Conclusions: The candidate miRNA can be chosen as a promising biomarker for predicting patient outcomes and even for early detection of the disease in clinical settings.   Oral squamous cell carcinoma, Hsa-miR-556-5p, real-time PCR 0 0 http://humangeneticsgenomics.ir/browse.php?a_code=A-10-362-1&slc_lang=en&sid=1 Behnaz Raei 1003194753284600450 1003194753284600450 No Department of Genetic, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran Yousef Seyedena 1003194753284600451 1003194753284600451 No Department of Genetic, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran Mojgan Alaeddini 1003194753284600452 1003194753284600452 No Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran Nazanin Hosseinkhan 1003194753284600453 1003194753284600453 No Endocrine Research Center, The Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran Mehrdad Hashemi 1003194753284600454 1003194753284600454 Yes Farhikhtegan Medical Convergence Sciences Research Center, Farhikhtegan Hospital, Tehran, Iran