en تخصصي Association study پژوهشي Research <div> <table align="left" hspace="0" vspace="0"> <tbody> <tr> <td style="padding: 0in 12px; text-align: justify;" valign="top"><span style="font-size:14px;"><span style="font-family:Times New Roman;"><span style="line-height:115%"><b><span style="line-height:115%"><span style="color:#0e101a">Background:</span></span></b><i><span style="line-height:115%"><span style="color:#0e101a"> Helicobacter pylori (H. pylori),&nbsp;</span></span></i><span style="line-height:115%"><span style="color:#0e101a">infecting half of the global population, undergoes genetic diversity. The aim of this study included&nbsp;<i>H. pylori</i>&nbsp;genotyping among patients referred to hospitals of Tehran using PCR-RFLP technique using&nbsp;<i>ureAB&nbsp;</i>and&nbsp;<i>ureCD</i>&nbsp;genes.&nbsp;</span></span></span><br> <span style="line-height:115%"><b><span style="line-height:115%"><span style="color:#0e101a">Materials and methods:</span></span></b><span style="line-height:115%"><span style="color:#0e101a">&nbsp;Identifying and genotyping such prevalent and variable pathogens is challenging. To date, two versions of&nbsp;<i>H. pylori&nbsp;</i>have been sequenced, indicating an immense variability in its genome. To distinguish the&nbsp;<i>H. pylori</i>&nbsp;subtypes, the PCR-RFLP technique was used as a differentiation method. Among 37&nbsp;<i>H. pylori-positive</i>&nbsp;gastric biopsies and 33 viable PCR products,&nbsp;<i>ureAB&nbsp;</i>and&nbsp;<i>ureCD</i>&nbsp;genes were selected for PCR-RFLP using&nbsp;<i>HaeIII&nbsp;</i>and&nbsp;<i>NdeII,&nbsp;</i>respectively. The resultant gel electrophoresis banding patterns were analyzed with visual evaluation.&nbsp;</span></span></span><br> <span style="line-height:115%"><b><span style="line-height:115%"><span style="color:#0e101a">Results:</span></span></b><span style="line-height:115%"><span style="color:#0e101a">&nbsp;PCR-RFLP standard run results were more appropriate compared to a single of each technique where a pair of&nbsp;<i>H. pylori&nbsp;</i>strains outlined identical banding patterns considering&nbsp;<i>UreAB/HaeIII</i>&nbsp;and&nbsp;<i>UreCD/NdeII&nbsp;</i>runs.&nbsp;</span></span></span><br> <span style="line-height:115%"><b><span style="line-height:115%"><span style="color:#0e101a">Conclusion:</span></span></b><span style="line-height:115%"><span style="color:#0e101a">&nbsp;The findings permit clinicians to make precise judgments on the priority of patients&#39; treatment, cost reduction, and mitigation of the trend of resistance emergence.&nbsp;</span></span></span></span></span></td> </tr> </tbody> </table> </div> Helicobacter pylori, Genotyping, PCR-RFLP technique 0 0 http://humangeneticsgenomics.ir/browse.php?a_code=A-10-367-2&slc_lang=en&sid=1 hadi alimadad hadi.alimadad2146@gmail.com 1003194753284600559 1003194753284600559 Yes payame noor kazem bidoki drbidoki@gmail.com 1003194753284600560 1003194753284600560 No payame noor dawud esmaili dawud.esmaili@gmail.com 1003194753284600561 1003194753284600561 No baghiatola abdolmajid ghasemian majidghasemian86@gmail.com 1003194753284600562 1003194753284600562 No medical sciences