Human Genetics and Genomics

en Piperine has anti-tumor effects in breast cancer by inducing mitochondrial apoptosis pathway تخصصي Gene expression پژوهشي Research <div style="text-align: justify;">Background: Breast cancer (BC) is the second leading cause of death due to cancer among women worldwide. Therefore, the present study investigates the cytotoxic effects of piperine on the breast cancer cell line (MCF7) and the genes of the apoptotic pathway.  Objectives: This research was performed to assess the effect of piperine on BC cells and the change in the expression level of bax gene through the induction of apoptosis. Methods: MCF-7 cells were prepared by the Pasteur Institute, Iran. Cytotoxicity of piperine at concentrations of (5, 10, 15, 20, and 25 µM) during 24, 48, and 72 hours was evaluated by MTT assay. The cell apoptosis and bax gene expression were evaluated by Flow Cytometry and qReal-time PCR, respectively. Finally, the statistical analysis of MTT and RT- PCR data was done by SPSS software version 22.  Results: The piperine showed concentration-dependent cytotoxic effects on the MCF-7 cell line in MTT assay. The bax gene expression level has a significant increase in piperine-treated cells compared to the untreated ones. The MCF-7 cell apoptosis at IC50 concentration of piperine was measured at 58.3% during 48-h treatment.  Conclusions: In general, it can be concluded that piperine has cytotoxic effects against breast cancer by inducing apoptosis via overexpressing of bax. </div> Piperine, Breast cancer, Apoptosis, MTT assay, Flow Cytometry. 0 0 http://humangeneticsgenomics.ir/browse.php?a_code=A-10-371-1&slc_lang=en&sid=1 Vahid Naseh vahid55vet@yahoo.com 1003194753284600752 1003194753284600752 Yes Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran Jalal Rezaeidian jalal_rezaeidian@yahoo.com 1003194753284600753 0009-0001-1934-7501 No Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran Maliheh Entezari entezarimali@yahoo.com 1003194753284600754 1003194753284600754 No Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran Hakimeh Ziyadi behnazziyadi@yahoo.com 1003194753284600755 1003194753284600755 No Department of Organic Chemistry, Faculty of Pharmaceutical Chemistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. Mehrdad Hashemi drmehashemi@gmail.com 1003194753284600756 1003194753284600756 No Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran