en تخصصي Gene expression پژوهشي Research <div> <table align="center" hspace="0" vspace="0"> <tbody> <tr> <td style="padding: 0cm 12px; text-align: justify;" valign="top"><span style="font-size:14px;"><span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><b><span style="line-height:115%"><span style="font-family:CapitoliumNewsW01-2Regular">Background: </span></span></b><span style="line-height:115%"><span style="font-family:CapitoliumNewsW01-2Regular">It is essential to find natural compounds with wound-healing properties that are accompanied by antibacterial effects. Therefore, in the present study, the wound-healing potential of curcumin dendrosomes on fibroblast cell line was evaluated.</span></span></span></span><br> <span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><b><span style="line-height:115%"><span style="font-family:CapitoliumNewsW01-2Regular">Methods</span></span></b><span style="line-height:115%"><span style="font-family:CapitoliumNewsW01-2Regular">: The HFF1 cell proliferation was evaluated by MTT test after 48 hours of curcumin (Cur) and curcumin dendrosomes (Cur.den) treatments. Scratching test was used to check cell migration and the expression levels of TNF-&alpha; and TGF-&beta; genes in cells were studied by Real-Time PCR technique. To investigate the antibacterial activity of Cur and Cur.den against S. epidermidis, well diffusion and microdilution tests were used.</span></span></span></span><br> <span style="line-height:115%"><span sans-serif="" style="font-family:Calibri,"><b><span style="line-height:115%"><span style="font-family:CapitoliumNewsW01-2Regular">Results</span></span></b><span style="line-height:115%"><span style="font-family:CapitoliumNewsW01-2Regular">: Cur.den improved significantly HFF1 cell proliferation and migration compared to both untreated control and Cur-treated cells. Moreover, this nanoformulation significantly overexpressed TGF-&beta; and downregulated TNF-&alpha; genes compared to control cells. Both Cur and Cur.den reduced S. epidermidis growth in well diffusion assay and their MICs were measured at 200 and 100 &micro;M, respectively. </span></span></span></span></span></td> </tr> </tbody> </table> </div> <div style="text-align: justify;"><span style="font-size:14px;"><b><span style="line-height:107%"><span style="font-family:CapitoliumNewsW01-2Regular">Conclusion</span></span></b><span style="line-height:107%"><span style="font-family:CapitoliumNewsW01-2Regular">: The Cur.den has a high potential to be used as a wound-healing drug. However, studies in animal models and clinical settings are recommended.</span></span></span></div> Curcumin, Dendrosome, Expression, Gene, Migration, Proliferation. 0 0 http://humangeneticsgenomics.ir/browse.php?a_code=A-10-370-2&slc_lang=en&sid=1 Siroos Tarighi siroosta2@gmail.com 1003194753284600735 0009-0001-5228-6085 No MSc, Department of Cellular and molecular biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran Marjan marjaninia arjanmarjaninia54@gmail.com 1003194753284600736 1003194753284600736 No MCs, Department of Biotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. Spiedeh khaleghi sp.khaleghi@gmail.com 1003194753284600737 1003194753284600737 No Ph.D, Department of Biotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran Majid Sadeghizadeh sadegh_maj@yahoo.com 1003194753284600738 1003194753284600738 No Ph.D, Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Maryam Montazeri maryam104@yahoo.com 1003194753284600739 1003194753284600739 Yes Assistant Professor, Department of Medical Biotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.