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Exploring the Effects of Magnetic Fields on miR-124, miR-195, and Their Target Gene CDK6 in Bladder Cancer Cells: Insights into miRNA-Mediated Gene Regulation
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چکیده: (10 مشاهده) |
Abstract
Background: Extremely low-frequency magnetic field (ELF-MF) is widespread environmental factors that may influence cellular behavior through non-thermal mechanisms. Recent evidence suggests that electromagnetic exposure can modulate gene expression and microRNA (miRNA) profiles in cancer cells. The aim of this research is the 50 Hz ELF-MF effect on the expression of miR-124, miR-195, and their target gene CDK6 in the 5637 bladder cancer cell line.
Methods: 5637 bladder cancer cells were cultured under standard conditions and exposed to ELF-MF for 6 hours per day over three consecutive days. The viability of cell was evaluated by MTT method. Total RNA extracted, and the change of expression of miR-124, miR-195, and CDK6 were quantified by quantitative real-time PCR.
Results: ELF-MF exposure could significant reduced of the viability of cell . The greatest reduction observed at 4 mT. Quantitative analysis revealed significant upregulation of miR-124 and miR-195 following magnetic field exposure, while CDK6 expression was significantly downregulated. Spearman correlation results demonstrated a negative correlation between miR-124/miR-195 and CDK6 expression and a positive correlation between miR-124 and miR-195.
Conclusion: Exposure to ELF-MF modulates miRNA expression and suppresses CDK6 , suggesting that magnetic fields can inhibit cell proliferation through miRNA-mediated regulation of the cell cycle. These findings provide insight into the interaction between physical environmental factors and gene regulatory networks in cancer biology.
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متن کامل [PDF 327 kb]
(7 دریافت)
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نوع مطالعه: پژوهشي |
موضوع مقاله:
عمومى
دریافت: 1404/11/24 | پذیرش: 1404/12/6 | انتشار: 1404/3/9
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