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Anti-apoptotic effect of biological synthesized Fe2O3 NPs on colorectal cancer cell line of HT-29 on PLA scaffold
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Saina Ghasemi   , Zeinab Piravar * , Mina Ramezani |
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Abstract: (9 Views) |
Nanoparticle (NP) based systems are developed to enhance drug delivery, reducing the toxic effect of medicine molecules. iron oxide nanoparticles are used for cancer treatment and have several advantages. For example, the ability to target specific locations and reduced side effects. This study aimed to investigate the therapeutic effect of colorectal cancer cells treated with biological synthesized Fe2O3 NPs with Prosopis farcta on polylactic acid scaffolds.
Materials and Methods
Colorectal cancer cell line HT-29 and normal cells HEK-293 were cultured on PLA scaffold and treated with biological synthesized Fe2O3 NPs for 48h. The influence of the treatment on the cells was examined by MTT and flow cytometry. To study the therapeutic mechanism of the Fe2O3 Bax, Bcl-2 and were detected by real-time polymerase chain reaction (RT-PCR).
Results
Fe2O3 NPs treatment (112 µg/ml) proved to have a good anticancer effect through the inhibition of tumoral cell proliferation and cellular growth significantly. It was shown that Fe2O3 could cause cellular necrosis, induce cellular apoptosis with reduced effects on normal colorectal cells.
Conclusion Our data showed that biological synthesized Fe2O3 increases significantly reduction of cancer cell viability with reduced side effect on normal cells.
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| Keywords: Fe2O3 NPs, Colorectal Cancer, Apoptosis, Bax, Bcl2. |
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Full-Text [PDF 2707 kb]
(10 Downloads)
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Type of Study: Research |
Subject:
Human Genetics
Received: 2025/06/4 | Accepted: 2025/08/22 | Published: 2024/08/20
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