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Piperine has anti-tumor effects in breast cancer by inducing mitochondrial apoptosis pathway
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Vahid Naseh *    , Jalal Rezaeidian , Maliheh Entezari , Hakimeh Ziyadi , Mehrdad Hashemi |
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Abstract: (370 Views) |
Background: Breast cancer (BC) is the second leading cause of death due to cancer among women worldwide. Therefore, the present study investigates the cytotoxic effects of piperine on the breast cancer cell line (MCF7) and the genes of the apoptotic pathway.
Objectives: This research was performed to assess the effect of piperine on BC cells and the change in the expression level of bax gene through the induction of apoptosis.
Methods: MCF-7 cells were prepared by the Pasteur Institute, Iran. Cytotoxicity of piperine at concentrations of (5, 10, 15, 20, and 25 µM) during 24, 48, and 72 hours was evaluated by MTT assay. The cell apoptosis and bax gene expression were evaluated by Flow Cytometry and qReal-time PCR, respectively. Finally, the statistical analysis of MTT and RT- PCR data was done by SPSS software version 22.
Results: The piperine showed concentration-dependent cytotoxic effects on the MCF-7 cell line in MTT assay. The bax gene expression level has a significant increase in piperine-treated cells compared to the untreated ones. The MCF-7 cell apoptosis at IC50 concentration of piperine was measured at 58.3% during 48-h treatment.
Conclusions: In general, it can be concluded that piperine has cytotoxic effects against breast cancer by inducing apoptosis via overexpressing of bax. |
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| Keywords: Piperine, Breast cancer, Apoptosis, MTT assay, Flow Cytometry. |
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Full-Text [PDF 782 kb]
(163 Downloads)
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Type of Study: Research |
Subject:
Gene expression
Received: 2023/10/26 | Accepted: 2024/02/29 | Published: 2024/03/1
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Ethics code: IR.IAU.VARAMIN.REC.1399.046
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