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Cell Death Induction and Bax gene expression of Cisplatin and Centaurea behen extract in human breast cancer cell line (MCF-7)
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Maliheh Entezari *   , Somaieh Sheikhan , Zeinab Khazaei Koohpar |
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Abstract: (3 Views) |
Background: The uncontrolled growth of the cells in the breast tissue causes breast cancer. About 1.38 new cases of breast cancer are diagnosed worldwide every year. Breast cancer is the most common cancer among women in the United States, with over 266,000 new cases expected for the year 2018. The evolution of breast cancer is a multi-stage process that involves genetic, epigenetic, and environmental factors that may interfere with the main settings of oncogenes and tumor suppressor genes, leading to the activation of signaling pathways associated with cancer that one of the most important of these pathways is the apoptosis process. The Bax gene also acts as an apoptotic stimulant.
Method: Due to the importance of this gene in the apoptotic process, in this research, the expression level of the Bax gene under treatment of cisplatin and Centaurea behen agents for 24 hours and 48 hours was investigated using the Real-time-PCR method.
Results: The results obtained illustrate that the expression level of this gene under treatment with the cisplatin and Centaurea behen has increased compared to the non-treatment state, so this expression increase showed a significant difference between the samples group and control group (P<0.05).
Conclusion: We have shown that the Centaurea behen extract and cisplatin could induce apoptosis in the MCF-7 cell line, over-expressing of the Bax gene.
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| Keywords: apoptosis, breast cancer, Bax, cisplatin, Centaurea behen, quantitative-PCR method |
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Full-Text [PDF 482 kb]
(4 Downloads)
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Type of Study: Research |
Subject:
Human Genetics
Received: 2024/06/12 | Accepted: 2025/04/23 | Published: 2024/05/21
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